yfiB [2018-05-04 17:47:23]

similar to [SW|ABC transporter] (ATP-binding protein)

locus: BSU08210

pI: 8.93

mw: 63.77 kDa

protein length: 573 aa

gene length: 1719 bp

function: unknown

product: [SW|ABC transporter] (ATP-binding protein)

essential: no

synonyms

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.2|Exporters] → [category|SW 1.2.1.2.6|Exporters of unknown function]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 893,904 → 895,625

The protein

Protein family

• [SW|ABC transporter] domain (according to Swiss-Prot)

[SW|Domains]

• has both a membrane-spanning and an ATP-binding domain [Pubmed|10092453]

Structure

• [PDB|3QF4] ([protein|288CA73D93B1E5106E1C6CE5E7E2E8BE2A6BD3F3|YfiB]-[protein|E85814EDF232D21A42380D559EAD2CDFB41F19DB|YfiC] complex from Thermotoga maritima, 44% identity) [pubmed|22447242]

[SW|Localization]

• cell membrane [Pubmed|10092453]

Expression and Regulation

Operons

• genes: [gene|288CA73D93B1E5106E1C6CE5E7E2E8BE2A6BD3F3|yfiB]-[gene|E85814EDF232D21A42380D559EAD2CDFB41F19DB|yfiC]
  description: [pubmed|22383849]

Biological materials

Mutant

• MGNA-C292 (yfiB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2290 NBRP B. subtilis, Japan]
• BKE08210 (Δ[gene|288CA73D93B1E5106E1C6CE5E7E2E8BE2A6BD3F3|yfiB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE08210 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTAAAAGCCTCCAAACC, downstream forward: _UP4_TTCGGAAGGGAGGGGAGCGA
• BKK08210 (Δ[gene|288CA73D93B1E5106E1C6CE5E7E2E8BE2A6BD3F3|yfiB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK08210 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTAAAAGCCTCCAAACC, downstream forward: _UP4_TTCGGAAGGGAGGGGAGCGA

References

• 10092453,22447242